Human Elite Gene Pooled shRNA Library

Amerigo Scientific offers high-quality premade pooled shRNA libraries targeting human and mouse genes. These RNAi libraries can serve as powerful and cost-efficient tools for large-scale loss-of-function screens across diverse biological processes.

Human gene targets
Elite Gene (~2,000 most frequently cited genes on PubMed Central)
Whole Genome (>20,000 RefSeq genes)

• Validation of library quality by NGS
The libraries undergo rigorous validation through next-generation sequencing (NGS), and consistently demonstrate outstanding performance with high alignment scores and uniformity. The meticulous validation process ensures that the libraries are not only accurate but also maintain a high degree of consistency across sequences.

• Genome-wide targeting and high complexity
For both human and mouse, we offer shRNA libraries at two scales: Whole Genome (~19,000 RefSeq genes) and Elite Gene (~2,000 most frequently cited genes on PubMed Central). On average, every gene is targeted by 5-6 different shRNAs with good knockdown scores calculated based on a set of rules following the guideline of the RNAi consortium (TRC). This enables more reliable knockdown and more effective screening. Detailed lists of target genes and shRNAs can be found under “Documents”.

• High uniformity
The pooled plasmid libraries have been validated by next-generation sequencing (NGS), which has successfully detected 100% of shRNAs in the Elite Gene libraries and >97% of shRNAs in the Whole Genome libraries. In addition, shRNA representation is highly uniform in the plasmid libraries.

• Well-established lentiviral vector and high titer lentivirus that is ready to use
The pooled shRNA libraries are expressed in the third-generation lentiviral vector system driven by human U6 promoter, which is a highly efficient system for stably knocking down expression of target genes in a wide variety of cells. This lentiviral vector system is ideal for in vitro genetic screens since it can introduce shRNAs into cells permanently, efficiently and uniformly. All pooled shRNA libraries are provided as ready-to-use lentivirus with high functional titer (>10^8 TU/ml), which saves your time in virus packaging and titer measurement. The third-generation lentiviral vector system is optimized for improved biosafety given its incompetent self-replication feature.

Please contact us at for specific academic pricing.