HiPure SF Plant DNA Kit

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.

Principle
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages
• Safe - require nophenol or chloroform extraction
• Fast - several samples can be extracted in 30 minutes by silica technology
• High purity - high quality DNA, completely remove inhibitors
• High yield - silica technology can achieve the highest yield

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