HiPure Microbial RNA Kit

Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages
• Fast - several samples can be extracted in 30 minutes
• High purity - the purified RNA can be directly used in various downstream applications
• High recovery - RNA can be recovered at the level of PG
• Good repeatability - silica gel column purification technology can obtain ideal results every time
• Broad spectrum - it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
• Sufficient components - the kit contains carried wall breaking enzymes and glass beads

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