HighPrep RNA Elite

Magnetic beads based reagent for manual and automated clean-up or concentration of RNA (including miRNA, siRNA, aRNA) and single-stranded cDNA after enzymatic reactions or prior sequencing.

The HighPrep RNA Elite utilizes the solid-phase paramagnetic bead-based technology for high-throughput purification of RNA or cDNA for in vitro applications such as transcription, antisense RNA (aRNA) amplification as well as for RNA and cDNA probe synthesis. This protocol enables recovery even of micro RNA (miRNA), small RNA and total RNA from enzymatic reactions, concentrating of miRNA and total RNA from a diluted sample. The HighPrep RNA Elite can be used for manual RNA clean-up as well as on automated liquid handling workstations.

Benefits
· Rapid and reliable clean-up of ss cDNA and RNA, including miRNA, siRNA, and aRNA
· Efficient purification of both large and small RNAs, and cDNA
· Complete removal of salts, unincorporated primers, and nucleotides
· No centrifugation step, no filtration step
· Adaptable to your current liquid handling workstation

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
· Add HighPrep RNA Elite reagent to sample
· Bind RNA to paramagnetic beads
· Separation of beads from contaminants
· Wash beads with 70% ethanol to remove contaminants
· Elute purified RNA or cDNA from beads

Please contact us at for specific academic pricing.