HighPrep DTR Paramagnetic Bead-based Reagent

Magnetic beads based reagent for manual and automated Sanger sequencing reaction clean-up for both ABI and MegaBACE sequencing platforms.

The HighPrep DTR is a high performance paramagnetic bead-based reagent for BigDye™ terminator removal from Sanger sequencing reactions for reliable and long readings of DNA sequences. The HighPrep DTR consists of a selective binding of DNA to the paramagnetic beads, followed with washing off nucleotides, primers, and non-targeted amplicons, and finally elution of pure DNA. HighPrep DTR is an excellent choice for both manual and fully automated purification of sequencing reaction products. The protocol can be adapted to common liquid handling workstation.

Benefits
· Rapid and reliable clean-up of sequencing reactions
· High DNA recovery yields, long sequence readings
· Flexible - can be used in any format
· No centrifugation step, no filtration step
· Adaptable to common liquid handling workstations

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
· Add HighPrep DTR reagent to sample
· Add freshly prepared 85% ethanol
· Bind sample to paramagnetic beads
· Wash beads with 85% ethanol to remove contaminants
· Elute purified DNA from beads

Please contact us at for specific academic pricing.