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Overview
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Our HiExpress™ firefly luciferase mRNA was generated with or without modified nucleotide, N1-Methylpseudouridine (m1Ψ). The firefly luciferase mRNA has been sequence optimized and fully validated for high levels of expression both in vitro and in vivo.
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Overview
(A) Expression of HiExpress™ Firefly Luciferase mRNA and other luciferase mRNA in HEK293T cells. Cells grown on a 12-well plate were transfected with 0.5 ug of mRNA per well and luciferase activity was measured at 6 h, 24 h, 48 h, and 72 h post transfection. (B) Luciferase activity measured in adult C57BL/6 mice injected intramuscularly with 30 ug of LNP encapsulated mRNA at 6 h, 24 h, and 48 h post injection. FLuc WT indicates wild-type firefly luciferase. FLuc WT (co) indicates codon-optimized wild-type firefly luciferase. FLuc2 indicates Luc2 firefly luciferase.
HiExpress™ Firefly Luciferase IVT mRNA (m1Ψ substitution)
HiExpress™ Firefly Luciferase IVT mRNA (m1Ψ substitution)
Catalog Number:
TR02457007VEC
Mfr. No.:
NR1021-0100; NR1021-1000
Price:
$798
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