HGF CHO Recombinant Protein

HGF CHO Recombinant Protein

Catalog Number:
P001418387ABE
Mfr. No.:
Abe32-1301
Price:
  • Size:
    10 µg
    Quantity:
    Add to Cart:
      • Overview
        • Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 75kDa. The HGF is purified by proprietary chromatographic techniques. Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3and GM-CSFto stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.

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      • Properties
        • Protein Name
          Scatter Factor (SF), Hepatopoietin (HPTA), HGF.
          Source
          CHO cells
          Type
          Recombinant Proteins
          Purification
          Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
          Formulation
          The protein was lyophilized from a concentrated solution containing Phosphate-Buffered Saline with 0.02% Tween 80, pH 7.4.
          Storage
          Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.

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