Name: HGF Sf9 Recombinant Protein
Price: Inquiry

- Overview
  - Hepatocyte Growth Factor Human Recombinant produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain containing 692 a.a and having a total molecular mass of 78.0 KDa. The HGF is purified by proprietary chromatographic techniques. Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.
    
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- Properties
  - Protein Name
    
    Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF.
    
    Source
    
    Insect cells
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 95.0% as determined by SDS-PAGE.
    
    Formulation
    
    The sterile protein powder (1 mg/ml) is lyophilized from a solution containing 50mM acetic acid.
    
    Storage
    
    Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in 50mM acetic acid to a concentration not less than 100µg/ml. Further dilutions should be made using buffer containing protein. The activity was assayed for scattering activity in the MDCK cell assay. The ED50 for this effect is typically at 1.0-5.0 ng/ml (200,000-1,000,000 IU/mg).

HGF Sf9 Recombinant Protein

HGF Sf9 Recombinant Protein

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