Heparin Catcher HC2 Coated - 96 well Solid plates

Heparin Catcher HC2 Plates 96-Well Solid Plates: Solid format designed for high-performance assays.

Heparin Catcher HC2 Plates are specifically designed for in vitro measurement of unfractionated heparin in low-protein fluids using ELISA assays. The specialized surface of these plates enables the immobilization and quantification of heparin in the range of 0.5 to 40.0 U/ml. Heparin, a widely studied glycosaminoglycan known for its anticoagulant properties, is used in both its native unfractionated form (UFH) and as low molecular weight (LMW) heparin. The HC2 heparin ELISA assays operate as competitive assays, where the colorimetric signal is inversely proportional to the heparin concentration in the sample, ensuring precise and reliable measurements.

Heparin Catcher HC2 Coated 96 Well Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Heparin Catcher HC2 Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Heparin Catcher HC2 surface is designed to immobilize heparin from fluid samples effectively. This product enables the measurement of heparin concentrations ranging from 0.5 to 40.0 U/ml, making it ideal for applications requiring accurate detection across this higher concentration range.

Key features of Heparin Catcher HC2 Plates
· Heparin Detection:It can detect concentrations ranging from 0.4 to 40.0 U/ml.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Heparin Catcher Coated Surface is designed for the in vitro measurement of unfractionated heparin in low protein content fluid by ELISA assays.
Heparin Catcher Coated Surface is a special surface to be able to immobilize and measure level heparin present in the fluids at different ranges of U/ml . Heparin is one of the most intensively studied glycosaminoglycans as a result of its anticoagulant properties; in facts, it is used as an anticoagulant either in its native unfractionated form (UFH – M.W. ~ 16 kDa) or as a partially depolymerized form called low molecular weight (LMW – M.W. ~ 4-8 kDa) heparin.

Heparin Assay
The proposed assays exploit the different Biomat Heparin Catcher Plates (HC1, HC2 or HC3), by quantitative enzyme-linked assays for the in vitro measurement of unfractionated heparin in low protein content fluid such as a buffer.

These heparin ELISA tests are competitive assays in which the colorimetric signal is inversely proportional to the amount of heparin present in the sample.

Principle of the assay
Samples to be assayed are at first mixed with a known amount of biotinylated heparin within the wells of Biomat Heparin Catcher Plate.

The Heparin in the sample competes with biotinylated heparin to bind to the binding sites of the heparin catcher plate.

After the removal of the unbound reagent and sample, a streptavidin-peroxidase conjugate is added to reveal the reaction.

The concentration in the sample is determined using a standard curve of known amounts of heparin.

· Heparin Catcher Coated Surfaces main features
Biomat Heparin Catcher Coated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

Also, Heparin Catcher Coated Products ensures:

Uniformity
The Heparin Catcher Coated Products show a CV% less than 5 when they are used as a catcher of heparin-biotin in an ELISA format, using Streptavidin-HRP as detector and TMB as substrate.

Storage and Stability
The Heparin Catcher Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Heparin Catcher Coated Microplates Specifications
Coating
HC1 (range 0.01 – 2.0 U/ml) is coated using 100 µl/well;
HC2 (range 0.5 – 40.0 U/ml) is coated using 100 µl/well;
HC3 (range 2.0 – 160.0 U/ml) is coated using 200 µl/well.