Name: Goat anti Rat IgE (Fc specific), conjugated with Biotin
Price: 440.00 USD

- Overview
  - Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
    
    This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Formulation
    
    Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
    
    Storage
    
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.
    
    Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
    
    Note
    
    This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
    
    * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
- Applications
  - Application Description
    
    ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting.

Datasheet

Goat anti Rat IgE (Fc specific), conjugated with Biotin

Goat anti Rat IgE (Fc specific), conjugated with Biotin

Background

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