Name: Goat anti Human secretory component (free and bound), conjugated with Horseradish peroxidase
Price: 570.00 USD

- Overview
  - Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal human milk, using a high electroendosmosis agar plate, free secretory component is precipitated in the alpha-2 region. This antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in human milk or other secretions.
    
    This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Conjugate
    
    HRP
    
    Storage
    
    The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
    
    Note
    
    This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
    
    * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
- Applications
  - Application
    
    ELISA; ICC; IHC; WB
    
    Application Description
    
    ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting.
- Reference
  - 1. Roos, K., Martinsson, K., Ziegelasch, M. et al. Circulating secretory IgA antibodies against cyclic citrullinated peptides in early rheumatoid arthritis associate with inflammatory activity and smoking. Arthritis Res Ther, 2016, 18; 119. https://doi.org/10.1186/s13075-016-1014-1
    
    2. Karin Roos Ljungberg, Vijay Joshua, Thomas Skogh, Anders Eklund, C. Magnus Skold, Reza Karimi, Sven Nyren, Anna Svard, Anca I. Catrina and Alf Kastbom. Secretory anti-citrullinated protein antibodies in serum associate with lung involvement in early rheumatoid arthritis.
    Rheumatology, 2020, 59; 852–859.

Datasheet

Goat anti Human secretory component (free and bound), conjugated with Horseradish peroxidase

Goat anti Human secretory component (free and bound), conjugated with Horseradish peroxidase

Background

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