Goat anti-Human IgM Coated - 96 well plates - 8 Well Strips on 12x8 frame

Goat Anti-Human IgM Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.

The Goat Anti Human IgM Plates are ideal to determine the presence and concentration of human IgM immunoglobulins in biological fluids, such as plasma and serum, tissue lysate, cerebral spinal fluid, saliva, urine, cell culture supernatant. The Goat Anti Human IgM plates are coated with goat Anti Human IgM Fc5µ chain. These plates are an ideal tool to carry out ELISA assays to determine the presence and concentration of human IgM immunoglobulins.

Goat Anti Human IgM Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Goat Anti Human IgM surface is coated with goat antibodies Anti IgM Fc5µ chain.

The Goat Anti-hIgM surface is developed to detect human IgM in biological fluids, such as plasma and sera, tissue lysate, cerebral spinal fluid, saliva, urine, cell culture supernatant.

An affinity purified goat anti human IgM specifically binds the Fc region of human IgM immunoglobulins, with minimal cross reaction to human IgG and IgA, mouse, bovine and rabbit serum proteins.

These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgM (H+L) detection antibody.

Key features of Goat Anti-Human IgM Plates
· Specific Goat Antibodies coating: Coated with Anti-IgM Fc5µ chain for selective and accurate detection of immunoglobulins.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Anti Human Immunoglobulins
Goat Anti Human IgG/IgM/IgA coated surface
Biomat's Goat Anti-Human IgG/IgM/IgA Coated Surfaces are engineered for the specific capture and quantification of human immunoglobulins in biological samples such as serum, plasma, urine, cerebrospinal fluid, saliva, and others.

Each surface is coated with affinity-purified goat anti-human antibodies, specifically targeting the Fc region of IgG (Fcγ), IgM (Fcμ), or IgA (Fcα). These surfaces are ideal for sandwich ELISA assays, where reliability, low background, and high sensitivity are essential.

· Surface Characteristics
Fc-Specific Binding
Each coating binds selectively to the Fc fragment of the target immunoglobulin:
IgG via γ chain
IgM via µ chain
IgA via α chain

High Specificity
Minimal cross-reactivity with other immunoglobulins and heterologous serum proteins (e.g. mouse, bovine, rabbit).

Reliable Quantification
Enables accurate detection of low-abundance human antibodies.

High Binding Affinity
Surfaces show strong and specific adsorption of target immunoglobulins, enhancing assay performance.

· Main Features of Biomat's Anti-Human IgG/IgM/IgA Coated Surface
Biomat Coated Plates with Anti human IgG/IgM/IgA are suitable with ELISA Assay Technology due to these features:
Ready to use
Manufactured under ISO:9001 guidelines
Made in pure polystyrene with low fluorescence
Post-coated (blocked) for low non specific binding and long-term stability
Alphanumeric coding for easy well recognition
Microplates comply with SBS standards and the design assures a good performance in automatic processing plant
Supplied with lot-specific Certificate of Quality
For Research Use Only
Also, Anti human IgG/IgM/IgA Coated 96-Well Plates ensure:

· Performance and Uniformity
When used as a capture surface in ELISA format (with HRP-conjugated anti-human antibodies and TMB substrate), Biomat's Anti-Human IgG/IgM/IgA surfaces exhibit:
Uniform coating across all wells
Coefficient of Variation (CV%) < 10%, ensuring assay reproducibility
High sensitivity, with detection limits well below 1 ng/well (varies by isotype)

· Storage and Stability
Store at 2–8°C unopened, in original packaging

Once opened, keep sealed with desiccant and use before the indicated expiration date

Available Fc-Specific Surface Variants
Biomat offers Anti-Human Coated Surfaces for the following immunoglobulin classes:
Anti-Human IgG (Fcγ)
High specificity for human IgG Fc region
Minimal cross-reactivity with IgA, IgM, and heterologous sera
Anti-Human IgM (Fcμ)
Binds human IgM Fc region with strong affinity
Low cross-reaction with IgG, IgA
Anti-Human IgA (Fcα)
Targets human IgA Fc region
Minimal interference from other Ig classes
Each coating is applied at 100 μL/well and optimized for high signal-to-background ratios in ELISA assays.

· Anti human IgG/IgM/IgA Coated Surfaces Product specifications
Goat Anti Human IgG Fcγ
An affinity purified goat anti human IgG specifically binds the Fc region of human IgG immunoglobulins, with minimal cross-reaction to human IgM and IgA, mouse, bovine and rabbit serum proteins.

These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgG (H+L)-HRP detection antibody.

Goat Anti Human IgG Fcγ Coated 96-Well Plates Product Specifications
Coating
Affinity purified goat anti human IgG Fcγ is coated using 100 μl/well.

Binding capacity
Microplate was saturated with human IgG at a concentration of 0.500 μg/ml (50 ng/well) in an ELISA format using goat anti human IgG (H+L)-HRP as detector and TMB as substrate.

Sensitivity
Human IgG was detected at a concentration significantly above background in an ELISA format using goat anti human IgG (H+L)-HRP as detector and TMB as substrate.

Goat Anti Human IgM Fcµ
An affinity purified goat anti human IgM specifically binds the Fc region of human IgM immunoglobulins, with minimal cross reaction to human IgG and IgA, mouse, bovine and rabbit serum proteins.

These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgM (H+L) detection antibody.

· Goat Anti Human IgM Fcµ Coated 96-Well Plates Product Specifications
Coating
Affinity purified Goat anti human IgM Fcµ is coated using 100 μl/well.

Binding capacity and Sensitivity
Microplate was saturated with human IgM at a concentration of about 3.0 μg/ml (300 ng/well) in an ELISA format using goat anti human IgM (H+L)-HRP as detector and TMB as substrate.

Sensitivity
Human IgM was detected at a concentration significantly above background in an ELISA format using goat anti human IgM (H+L)-HRP as detector and TMB as substrate.

Goat Anti Human IgA Fcα
An affinity purified goat anti human IgA specifically binds the Fc region of human IgA immunoglobulins, with minimal cross reaction to human IgG and IgM, mouse, bovine and rabbit serum proteins.

These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgA (H+L) detection antibody.

· Goat Anti Human IgA Fcα Coated 96-Well Plates Product Specifications
Coating
Affinity purified Goat anti human IgA Fcα is coated using 100 μl/well.

Binding capacity
Microplate was saturated with human IgA at a concentration of about 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti human IgA (H+L)-HRP as detector and TMB as substrate.

Sensitivity
Human IgA was detected at a concentration significantly above background in an ELISA format using goat anti human IgA (H+L)-HRP as detector and TMB as substrate.