Name: GMBS
Price: 313.00 USD

- Overview
  - Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Hetero-bifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cross-Linking Reagents
    
    CAS Number
    
    80307-12-6
    
    Molecular Formula
    
    C12H12N2O6
    
    Molecular Weight
    
    280.23
    
    Appearance
    
    White powder
    
    Purity
    
    ≥95.0% (HPLC)
    
    Storage
    
    0-5°C
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Reference
  - 1) S. Yoshitake, Y. Yamada, E. Ishikawa and Rene Masseyeff, Conjugation of Glucose Oxidase from Aspergillus Niger and Rabbit Antibodies Using N-Hydroxysuccinimide Ester of N-(4-Carboxycyclohexylmethyl)-Maleimide, Eur. J. Biochem., 1979, 101, 395.
    
    2) H. Tanimori, T. Kitagawa, T. Tsunoda and R. Tsuchiya, Enzyme Immunoassay of Neocarzinostain Using β-Galactosidase as Label, J. Pharm. Dyn., 1981, 4, 812.
    
    3) K. Fujiwara, M. Yasuno and T. Kitagawa, Novel Preparation Method of Immunogen for Hydrophobic Hapten, Enzyme Immunoassay for Daunomycin and Adriamycin, J. Immunol. Methods, 1981, 45, 195.
    
    4) T. Miura, H. Kouno and T. Kitagawa, Detection of Residual Penicillin in Milk by Sensitive Enzyme Immunoassay, J. Pharm. Dyn., 1981, 4, 706.
    
    5) S. Yoshitake, M. Imagawa, E. Ishikawa, Y. Niitsu, I. Urushizaki, M. Nishiura, R. Kanazawa and H. Ogawa, Mild and Efficient Conjugation of Rabbit Fab'-and Horseradish Peroxidase Using a Maleimide Compound and Its Use for Enzyme Immunoassay, J. Biochem. ,1982, 92, 1413.
    
    6) T. Kitagawa, H. Tanimori, K. Yoshida, H. Asada, T. Miura and K. Fujiwara, Studies on Viomycin. XV. Comparative Study on the Specificities of Two Anti-viomycin Antisera by Enzyme Immunoassay, Chem. Pharm. Bull., 1982, 30, 2487.
    
    7) T. Kitagawa, T. Kawasaki and H. Munechika, Enzyme Immunoassay of Blasticidin S with High Sensitivity:A New and Convenient Method for Preparation of Immunogenic(Hapten-Protein) Conjugates, J. Biochem.,1982, 92, 585.

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