Name: EMCS
Price: 313.00 USD

- Overview
  - Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Hetero-bifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cross-Linking Reagents
    
    CAS Number
    
    55750-63-5
    
    Molecular Formula
    
    C14H16N2O6
    
    Molecular Weight
    
    308.29
    
    Appearance
    
    white or slightly yellow powder
    
    Purity
    
    ≥90.0% (HPLC)
    
    Storage
    
    0-5°C
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Reference
  - 1) S. Yoshitake, Y. Yamada, E. Ishikawa and R. Masseyeff, Conjugation of Glucose Oxidase from Aspergillus Niger and Rabbit Antibodies Using N-Hydroxysuccinimide Ester of N-(4-Carboxycyclohexylmethyl)-Maleimide, Eur. J. Biochem., 1979, 101, 395.
    
    2) T. Kitagawa, T. Shimozono, T. Aikawa, T. Yoshida and H. Nishimura, Preparation and Characterization of Hetero-bifunctional Cross-linking Reagents for Protein Modifications, Chem. Pharm. Bull., 1981, 29, 1130.
    
    3) S. Yoshitake, M. Imagawa, E. Ishikawa, Y. Niitsu, I. Urushizaki, M. Nishimura, R. Kanazawa, H. Kurosaki, S. Tachibana, N. Nakazawa and H. Ogawa, Mild and Efficient Conjugation of Rabbit Fab'-and Horseradish Peroxidase Using a Maleimide Compound and Its Use for Enzyme Immunoassay, J. Biochem., 1982, 92, 1413.
    
    4) E. Ishikawa, M. Imagawa, S. Hashida, S. Yoshitake, Y. Hamaguchi and T. Ueno, Enzyme-Labelling of Antibodies and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining, J. Immunol., 1983, 4, 209.
    
    5) I. Weeks, A. K. Campbell and J. S. Woodhead, Two-site Immunochemiluminometric Assay for Human α1-Fetoprotein, Clin. Chem., 1983, 29, 1480.
    
    6) S. Hashida, M. Imagawa, S. Inoue, K. Ruan and E. Ishikawa, More Useful Maleimide Compounds for the Conjugation of Fab'-to Horseradish Peroxidase through Thiol Groups in the Hinge, J. Appl. Biochem., 1984, 6, 56.
    
    7) S. Inoue, M. Imagawa, S. Hashida, K. Ruan and E. Ishikawa, A Small Scale Preparation of Affinity-Purified Rabbit Fab'-Horseradish Peroxidase Conjugate for Enzyme Immunoassay, Anal. lett., 1984, 17, 229.
    
    8) M. Koizumi, K. Endo, M. Kunimatsu, H. Sakahara, T. Nakashima, Y. Kawamura, Y. Watanabe, T. Saga, J. Konishi, T. Yamamura, S. Hosoi, S. Toyama, Y. Arano and A. Yokoyama, Ga-Labeled Antibodies for Immunoscintigraphy and Evalution of Tumor Targeting of Drug-Antibody Conjugatesin Mice, Cancer Res., 1988, 48, 1189.
    
    9) T. Kohno and E. Ishikawa, Novel Enzyme Immunoassay(Immune Complex Transfer Enzyme Immunoassay) for Anti-insulin IgG in Guinea Pig Serum, Anal. lett., 1988, 21, 1019.

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