Glyceryl CPG 500A

Branching Amidites & 3'-Spacer Supports

This material is sensitive to heat and moisture. It requires the fastest shipment possible.

Branching phosphoramidites and solid supports:
Branched oligonucleotides are synthesized from the incorporated glycerol backbone, which possesses two protected hydroxyl groups for chain elongation. The branching phosphoramidites incorporate branching sites into an oligonucleotide. Branched oligonucleotides have been used effectively for signal amplification by introducing networks of labels or probing sites.

Symmetric branching phosphoramidites:

This reagent allows simultaneous extension from the glycerol branch point because both hydroxyl groups are protected by DMTr groups and are thus removed at the same time, resulting in two identical branches. Several cycles of incorporation of this amidite will result in a tree-like structure of two branched oligonucleotide arms. Since reporter molecules, such as biotin or fluorescein, can be attached to each arm, this exponential branching can amplify and thus improve the sensitivity of these reporter molecules.

Asymmetric branching phosphoramidites:

This reagent uses DMTr to protect one hydroxyl of the glyceryl backbone and a levulinyl group to protect the other. The DMT group is removed during the normal deblock step and routine chain elongation can continue from this branch. Subsequent removal of the levulinyl protecting group with hydrazine permits the elongation from the other, resulting in asymmetrical branching. The branches can differ in sequence, reporter molecules, or both.