Application Description
DNA PURIFICATION:
We offer GENE-SYS 1 solid phase DMT-ON purification/extraction 96 well micro purifier to enable parallel purification of synthetic oligonucleotides for 5 OD (260nm) to 50 OD (260 nm) quantities. The 96 position GENE-SYS 1 accommodates the polypropylene 0.7 mL/well, 1.2 mL/well, 1.25 mL/well, and 2.25mL/ well removable well filter plates. The system charges crude oligos followed by washing and elution without a cumbersome valve network.
GeneSys-12 (catalog #GS-1154) purifies up to 12 oligos at the same time.
GeneSys-24 (Catalog #GS-1158) purifies up to 24 oligos at the same time.
Puri-Pak PlateTM (Catalog # 3249) is a 96-Well purification extraction plate. Each well is filled with a unique packing material for oligo purification/cleanup. Each well is capable of the following:
DMT-ON purification of up to 1 μm scale per well, or DMT-OFF desalting of up to 25 OD per well.
GeneSys Manifold for the 96 Well format comes complete with a gasket for top and bottom collection plates. The system also includes a vacuum controller for low vacuum evacuation of the system for the 96-well solvent, buffer exchange, and dropwise collection of the purified oligo. This system has the advantage of simultaneous purification of large numbers of oligos in a 96-well purification format. The purification of the crude synthetic oligos can be achieved either by desalting of the crude synthetic oligo or by DMT-ON purification protocol of the crude synthetic DNA.
Besides the natural synthetic DNA oligonucleotides, the Puri-Pak Cartridges or the Puri-Pak plate can be used to purify terminal and nucleobase modified oligos, biotin, other chromophore labeled oligos, 2’-O-Methyl-oligos, and phosphorothioate labeled oligos.
96-Well collection plates (catalog # GS3280) have a unique design in which the purification plate fits in effectively in each well for the collection plate and accommodates a 2 ml volume per well.