Name: GB1 - ²H¹³C¹⁵N microcrystals
Price: Inquiry

- Overview
  - MW = 6.2 kDa calculated. GB1 (Immunoglobulin G-binding protein G, IgG-binding protein G) expressed in E. coli. The protein consists β-1 Immunoglobulin Binding Domain of Protein G, residues 303-357 (UniProtKB accession P19909), with the mutation T303Q (T2Q). This mutation reduces the cleavage of the N-terminal Met residue.
    
    · Characteristics
    Under the above described conditions, to avoid precipitation or protein aggregation, the product can be concentrated up to 3 mM.
    
    Labeling: 2H13C15N
    NMR Standard: Bulk sample
    Physical state: Microcrystalline
    Shipping in Dry Ice: no
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Streptococcus sp
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.
    
    Formulation
    
    1.0 mg/mL solution in sodium phosphate 20 mM, pH 7. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 9970 M^-1cm^-1 calculated).
    (Unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
    
    Storage
    
    -20°C. The protein is stable at 4°C for months at 25°C for at least two weeks. Avoid repeated freeze/thaw cycles.
    NMR USE: after the experiments return the sample at 4°C.
    
    Molecular Weight
    
    6.2 kDa
    
    Expression system
    
    E. coli
    
    Sequence
    
    310 320 330 340 350
    M-QYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT
    
    KTFTVTE
    
    * This product is intended for research use or further manufacturing only.

GB1 - ²H¹³C¹⁵N microcrystals

GB1 - ²H¹³C¹⁵N microcrystals

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