Name: L-Asparaginase 2 - ¹⁵N
Price: 1046.00 USD

- Overview
  - MW= 140 kDa calculated (tetramer). L-Asparaginase II from E. coli (ANSII) consists of a homotetrameric assembly of 140kDa with D2 symmetry. (residues 7-348, UniProtKB accession P00805).
    
    · Characteristics
    Under the above described conditions, the product can be concentrated up to 3 mM.
    
    Shipping in Dry Ice: no
    Labeling: 15N
    NMR Standard: Bulk sample
    Physical state: Solution
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight at about 35.0 kDa.
    
    Formulation
    
    1 mg/mL solution in phosphate buffer 150 mM pH 7.5. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 23505 M^-1cm^-1 calculated for the monomer).
    (Unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
    
    Storage
    
    -20°C. The protein is stable at 4°C for months. Avoid repeated freeze/thaw cycles.
    NMR USE: after the experiments return the sample at 4°C.
    
    Molecular Weight
    
    138 kDa (tetramer)
    
    Sequence
    
    10 20 30 40 50
    TALA ALVMGFSGAA LALPNITILA TGGTIAGGGD SATKSNYTVG
    60 70 80 90 100
    KVGVENLVNA VPQLKDIANV KGEQVVNIGS QDMNDNVWLT LAKKINTDCD
    110 120 130 140 150
    KTDGFVITHG TDTMEETAYF LDLTVKCDKP VVMVGAMRPS TSMSADGPFN
    160 170 180 190 200
    LYNAVVTAAD KASANRGVLV VMNDTVLDGR DVTKTNTTDV ATFKSVNYGP
    210 220 230 240 250
    LGYIHNGKID YQRTPARKHT SDTPFDVSKL NELPKVGIVY NYANASDLPA
    260 270 280 290 300
    KALVDAGYDG IVSAGVGNGN LYKSVFDTLA TAAKTGTAVV RSSRVPTGAT
    310 320 330 340
    TQDAEVDDAK YGFVASGTLN PQKARVLLQL ALTQTKDPQQ IQQIFNQY
    
    * This product is intended for research use or further manufacturing only.
- Reference
  - Structural characterization of a protein adsorbed on aluminum hydroxide adjuvant in vaccine formulation
    Characterization of PEGylated Asparaginase: New Opportunities from NMR Analysis of Large PEGylated Therapeutics.
    Characterization of the Conjugation Pattern in Large Polysaccharide-Protein Conjugates by NMR Spectroscopy

L-Asparaginase 2 - ¹⁵N

L-Asparaginase 2 - ¹⁵N

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