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Overview
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Fungus-Derived Ribonuclease T1 Animal Free Purified Lyophilized from Innovative Research has been prepared as a highly purified, microbial (non-mammalian) RNase prepared with non-animal components. This is a lyophilized powder with a concentration of 300,000 units/mg protein.
This product is a non-mammalian endoribonuclease, specifically for the cleavage of RNA (deaminated RNA) between guanosine 3’-phosphate residues (or inosine 3’-phosphate) and the 5’-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2’, 3’-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3’-phosphate termini. This product has multiple applications in molecular cloning and DNA sequencing. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X mM. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations.Please contact us at for specific academic pricing.
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- Properties
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Overview
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