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Overview
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eSpCas9 (enhanced specificity Cas9) has enhanced specificity compared to wild type Cas9 for CRISPR/Cas9 genome editing. eSpCas9 is able to reduce off-target effects and maintain robust on-target genome editing efficiency, allowing more efficient gene targeting. eSpCas9 (EF1a, Neo) lentivirus expresses eSpCas9 under the control of the EF1a promoter with neomycin as a selection marker, allowing selecting stable cell clones with neomycin.
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