Erythromycin A, EvoPure®

- Overview
  - Erythromycin A, EvoPure® is a highly purified form of the macrolide antibiotic Erythromycin A and is a natural product. Erythromycin is composed of Erythromycin A, B, C, D, and additional impurities. Erythromycin A is the most active component.
    
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    Background
    
    Macrolide antibiotics inhibit bacterial growth by targeting the 50S ribosomal subunit of the 70S ribosome preventing aminoacyl translocation of the A-site to P-side and peptide bond formation during protein synthesis. Resistance to Erythromycin is commonly attributed to mutations in 50S rRNA preventing erythromycin binding allowing the cell to synthesize proteins free of error. In acidic aqueous media, Erythromycin A is degraded via intramolecular dehydration to form erythromycin-6,9-hemiketal and then anhydroerythromycin, both of which possess little antimicrobial activity. (Fiese and Staffen, 1990).
- Properties
  - CAS Number
    
    59319-72-1
    
    Molecular Formula
    
    C37H67NO13 · 2H2O
    
    Molecular Weight
    
    769.96
    
    Other Properties
    
    Source: Saccharopolyspora erythraea
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Effective against Gram-positive and Gram-negative bacteria.
    
    Eukaryotic Cell Culture Applications: The production of bioactive compounds in heterologous hosts has become a valuable tool for the investigation of biosynthetic pathways. The reconstruction of the Erythromycin A biosynthetic pathway was done in E. coli (Zhang et al, 2010). The biosynthesis of 6-deoxyerythronolide B (6-dEB), the macrocyclic core of Erythromycin A, in an engineered strain of E. coli was described (Pfeifer et al., 2001).