EnzyFluo™ NAD/NADH Assay Kit

- Overview
  - Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Our EnzyFluo™ NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.
    
    Key Features
    ▪ Sensitive and accurate. Detection limit of 0.02 µM and linearity up to 1 µM NAD+/NADH in 96-well plate assay.
    ▪ Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.
    ▪ High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Method: F530/585nm
    Samples: Cell, tissue extracts etc Direct NAD/NADH Assays in 96-Well PlateNEW!!!
    Species: All
    Procedure: 10 min
    Detection Limit: 0.02 µM
    Shelf Life: 6 months
    
    Storage
    
    -20°C
    
    * For Research Use Only
- Applications
  - Application Description
    
    For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
- Reference
  - Chaurasiya, A., et al. (2021). Pathogen induced subversion of NAD+ metabolism mediating host cell death: a target for development of chemotherapeutics. Cell Death Discovery 7(1): 10. Assay: NAD and NADH in microbial cells.
    Joe, Y., et al. (2020). Cross-talk between CD38 and TTP is essential for resolution of inflammation during microbial sepsis. Cell Reports 30(4): 1063-1076.e5. Assay: NAD and NADH in mouse cells.
    Zhang, M., et al. (2020). Dysregulated metabolic pathways in age-related macular degeneration. Scientific Reports 10(1): 2464. Assay: NAD and NADH in human cells.
    Liemburg-Apers, Dania C., et al (2016). Acute stimulation of glucose influx upon mitoenergetic dysfunction requires LKB1, AMPK, Sirt2 and mTOR-RAPTOR. J Cell Sci 129.23: 4411-4423. Assay: NAD/NADH in mouse cells.
    Kim, Ha-Neui, et al. (2015) Sirtuin1 suppresses osteoclastogenesis by deacetylating FoxOs. Molecular Endocrinology 29.10: 1498-1509. Assay: NAD/NADH in mice.

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EnzyFluo™ NAD/NADH Assay Kit

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