EnzyFluo™ NFKB Phosphorylation Assay Kit

Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-α. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcription. Our cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.Haven’t Found the ELISA Kit for Your Target. We offer two DIY kits: Custom Cell-based ELISA Kit (EFC1M) for mouse antibody and Custom Cell-based ELISA Kit (EFC2R) for rabbit antibody. All that is required is a primary antibody.

Key Features
▪ New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs).
▪ Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary.
▪ Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

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