EnzyChrom™ Glucose Oxidase Assay Kit

Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery. Our glucose oxidase assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample.

Key Features
▪ Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate for 20 minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays.
▪ Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Please contact us at for specific academic pricing.