EnzyChrom™ Glutamate Assay Kit

- Overview
  - Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimer’s disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable. Our EnzyChrom™ glutamate assay kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample.
    
    Key Features
    ▪ Sensitive and accurate. Detection limit of 50 µM, linearity up to 2.5 mM glutamate in 96-well plate assay.
    ▪ Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed.
    ▪ High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Method: OD565nm
    Samples: Serum, plasma, urine, cell, tissue etc
    Species: All
    Procedure: 30 min
    Detection Limit: 50 µM
    Shelf Life: 6 months
    
    Storage
    
    -20°C
    
    * For Research Use Only
- Applications
  - Application Description
    
    For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism.
- Reference
  - Wray, R et al. (2020). Novel MscL agonists that allow multiple antibiotics cytoplasmic access activate the channel through a common binding site. PLOS ONE, 15(1), e0228153. Assay: Glutamate in E. coli.
    Potter, AD et al. (2020). Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection. Proceedings of the National Academy of Sciences, 117(22): 12394-12401. Assay: Glutamate in mouse bones.
    Rashmi, KS et al. (2020). The regulatory role of neurotransmitters in orexin b induced nucleus accumbens dependent alcohol ingestion in Wistar albino rats. RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY, 13(12): 6224-6230. Assay: Glutamate in mouse.
    Kansakar, U. (2019). Development of an Astrocyte/Glioma Co-Culture System for Measuring Cellular Dynamics. Assay: Glutamate in rat cells.
    Izrael, Michal, et al (2018). Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1 G93A and NSG animal models. Stem cell research & therapy 9.1: 152. Assay: Glutamate in human cells.
    Das, Champak, et al (2017). Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe. Sensors and Actuators B: Chemical 238: 633-640. Assay: Glutamate in glucose.
    Swiercz, Rafal, et al (2017). Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption. Oncotarget 8.2: 3528-3541. Assay: Glutamate in human cells.
    Izrael, Michal, et al (2016). Directed differentiation of astrocytes from human pluripotent stem cells for use in drug screening and the treatment of amyotrophic laterial sclerosis (als). U.S. Patent Application No. 15/025,946. Assay: Glutamate in human cells.
    Kahlert, Ulf D., et al (2016). Alterations in cellular metabolome after pharmacological inhibition of N otch in glioblastoma cells. International journal of cancer 138.5: 1246-1255. Assay: Glutamate in human cells.
    Wray, Robin, et a (2016). Dihydrostreptomycin directly binds to, modulates, and passes through the MscL channel pore. PLoS biology 14.6: e1002473. Assay: Glutamate in E. coli cells.
    Mesci, P et al (2015). System xC- is a mediator of microglial function and its deletion slows symptoms in amyotrophic lateral sclerosis mice. Brain. 138(Pt 1):53-68. Assay: Glutamate in mice spinal cord tissue.
    Iscla, I et al (2014). Streptomycin potency is dependent on MscL channel expression. Nature Communications. 5:4891. Assay: Glutamate in E. coli vector.
    Ramos, C et al (2012). Native Presynaptic Metabotropic Glutamate Receptor 4 (mGluR4) Interacts with Exocytosis Proteins in rat Cerebellum. Journal of Biological Chemistry 287(24):20176-86. Assay: Glutamate in rat protein lysate.
    Liang, Y., et al. (2011). Additive effect of tetramethylpyrazine and deferoxamine in the treatment of spinal cord injury caused by aortic cross-clamping in rats. Spinal Cord 49(2):302-6. Assay: Glutamate in rat.
    Rink, C., et al (2011). Oxygen-inducible glutamate oxaloacetate transaminase as protective switch transforming neurotoxic glutamate to metabolic fuel during acute ischemic stroke. Antioxid Redox Signal 14(10):1777-85. Assay: Glutamate in rat brain tissue.
    Yang, LM and Blount, P. (2011). Manipulating the permeation of charged compounds through the MscL nanovalve. FASEB J 25(1):428-34. Assay: Glutamate in E. coli.

MSDS

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Protocol

EnzyChrom™ Glutamate Assay Kit

EnzyChrom™ Glutamate Assay Kit

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