EnzyChrom™ Creatine Assay Kit

- Overview
  - CREATINE is present in vertebrates and helps to supply energy to muscle. In humans and animals, approximately half of creatine originates from food (mainly from fresh meat). Creatine supplementation has been investigated as a possible therapeutic approach for the treatment of muscular, neuromuscular, neurological and neurodegenerative diseases. Simple, direct and automation-ready procedures for measuring creatine are popular in research and drug discovery. Our creatine assay is based on enzymatic reactions leading to formation of a pink colored product. The optical density at 570 nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to the creatine concentration in the sample.
    
    Key Features
    ▪ High sensitivity and wide linear range. Use 10 µL sample. Linear detection range 4 to 1000 µM (colorimetric) or 0.5 to 50 µM (fluorimetric).
    ▪ Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of creatine within 30 minutes.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Method: OD570nm, or FL530/590nm
    Samples: Serum, plasma, urine, saliva etc
    Species: All
    Procedure: 30 min
    Detection Limit: 4 µM
    Shelf Life: 12 months
    
    Storage
    
    -20°C
    
    * For Research Use Only
- Applications
  - Application Description
    
    For quantitative determination of creatine and evaluation of drug effects on creatine metabolism.
- Reference
  - Wang, Yujie, et al (2018). Guanidinoacetic Acid Regulates Myogenic Differentiation and Muscle Growth Through miR-133a-3p and miR-1a-3p Co-mediated Akt/mTOR/S6K Signaling Pathway. International journal of molecular sciences 19.9: 2837. Assay: Creatine in murine cells.
    Chan, Yiumo Michael, et al (2017). Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model. PloS one 12.3: e0173261. Assay: Creatine in human and mouse tissues.
    Holz, Josefin-Beate, and Alex Hemeryck (2017). Inhibition of bone resorption with rankl binding peptides. U.S. Patent Application No. 15/295,229. Assay: Creatine in human urine.
    Schoborg, JA et al (2014). Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis. Biotechnology Journal 9(5): 630-640. Assay: Creatine in yeast cells.
    Qi S et al (2012). Comparison of the metabolic profiling of hepatitis B virus-infected cirrhosis and alcoholic cirrhosis patients by using (1) H NMR-based metabonomics. Hepatol Res 42(7):677-85. Assay: Creatine in human serum.

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Protocol

EnzyChrom™ Creatine Assay Kit

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