EnzyChrom™ D-Amino Acid Assay Kit

D-AMINO-ACIDS are not as widespread as their enantiomeric counterparts in proteins but they can be found in organisms ranging from bacteria (cell walls and antibiotics) to mammals (central nervous systems). The presence of D-amino acids in food is also of considerable interest. Racemization of L-amino acids during food processing may affect food quality and nutritional value. Our D-amino acid assay uses an enzyme-catalyzed oxidation of D-amino acids to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the D-amino acid concentration in the sample.

Key Features
▪ Fast and sensitive. Linear detection range: 0.86 to 500 µM (colorimetric assay) and 0.18 to 50 µM (fluorimetric assay) for 60 min reaction.
▪ Convenient. The procedure involves adding a single working reagent and reading after 60 minutes.
▪ High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to process thousands of samples per day.

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