EnzyChrom™ Choline Assay Kit

- Overview
  - CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. Our method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.
    
    Key Features
    ▪ Use 20 µL samples. Linear detection range: colorimetric assay 1 to 100 µM, fluorimetric assay 0.2 to 10 µM choline.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Method: OD570nm, or FL530/585nm
    Samples: Serum, plasma, urine, saliva, milk, tissue, cells etc
    Species: All
    Procedure: 30 min
    Detection Limit: OD, FL: 1, 0.2 µM
    Shelf Life: 6 months
    
    Storage
    
    -20°C
    
    * For Research Use Only
- Applications
  - Application Description
    
    For quantitative determination of choline and evaluation of drug effects on choline metabolism.
- Reference
  - Winnard Jr, PT et al (2020). Brain metabolites in cholinergic and glutamatergic pathways are altered by pancreatic cancer cachexia. Journal of Cachexia, Sarcopenia and Muscle, 11(6), Assay: Choline in mouse plasma.
    Ballester-Lozano, GF et al (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma.
    Benedito-Palos, L et al (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18. Assay: Choline in fish plasma.
    Ballester-Lozano, GF et al (2014). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma.
    Strilakou, AA et al (2014). Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats. Canadian Journal of Physiology and Pharmacology 92(1): 78-84. Assay: Choline in rat serum.

MSDS

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Protocol

EnzyChrom™ Choline Assay Kit

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