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Overview
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AMYLASE belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and “limit dextrin” from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure. Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. Our EnzyChrom™ a-amylase assay method involves two steps: (1). α-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen peroxide concentration is determined with a colorimetric reagent.
Key Features
▪ Sensitive and accurate. Linear detection range 0.3 to 50 U/L α-amylase in 96-well plate assay.
▪ Convenient. The procedure involves adding a single working reagent, incubation for 15 min, followed by the detection reagent and a 20-min incubation and reading the optical density at 585 nm.Please contact us at for specific academic pricing.
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- Properties
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Overview
EnzyChrom™ α-Amylase Assay Kit
EnzyChrom™ α-Amylase Assay Kit
Catalog Number:
HTSA373901BIO
Mfr. No.:
BIO-ECAM-100
Price:
$668
Size:
100 tests
Quantity:
Add to Cart:
.