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Overview
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DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).
Features
• Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.Please contact us at for specific academic pricing.
More Details
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- Properties
- Applications
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Overview