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Overview
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This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Features
• lack of Lon and Omp proteases allows high-level protein expression and accumulation
• Easy gene inductionPlease contact us at for specific academic pricing.
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