ECOS™ 21 Competent Cells [BL21(DE3)]

ECOS™ 21 Competent Cells [BL21(DE3)]

Catalog Number:
CC02547411YEA
Mfr. No.:
FYE207-5VL/40VL/96WL
Price:
Inquiry
  • Size:
    Quantity:
    Add to Cart:
    Add to Cart
      • Overview

        • This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.

          Features
          • lack of Lon and Omp proteases allows high-level protein expression and accumulation
          • Easy gene induction

          Please contact us at for specific academic pricing.

          More Details

          ECOS™ Competent Cells with High Transformation Efficiencies
      • Properties
        • Other Properties
          Efficiency: >2 x 10⁷ cfu/μg
          Control Plasmid (pUC19): 5μl (10⁻⁴ μg/μl)
          Genotype: F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
          Storage
          -70°C

          * For Research Use Only.

      • Applications
        • Application Description
          • IPTG induced gene expression in E. coli
          • Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
          • Subcloning

    Documents

    Manual

    ECOS Card

    We Also Recommend

    ECOS™ 9-5 Competent Cells [JM109]

    ECOS™ 9-5 Competent Cells [JM109]

    ECOS™ Economic 9-5 Competent Cells [JM109]

    ECOS™ Economic 9-5 Competent Cells [JM109]

    ECOS™ Super 9-5 Competent Cells [JM109]

    ECOS™ Super 9-5 Competent Cells [JM109]