ECOS™ 9-5 Competent Cells [JM109]

JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA and the production of ssDNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.

This product can become a powerful cloning tool when it is used in combination with the rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

Features
• Rapid growing strain
• Carries F’ episome

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