E.coli HCP ELISA Kit (Plasmid)

The use of Escherichia coli (E. coli) for expression and amplification in the production of recombinant proteins and plasmid DNA is a relatively simple and cost-effective way to prepare biological products. However, the process using E. coli will inevitably introduce host cell protein (HCP) impurities, which will reduce the efficacy of the product and may cause adverse toxicity or immune reactions. Therefore, it is necessary to ensure that the residual level of HCP in the final product of the biological product is as low as possible.
ELISA is a simple, sensitive, objective and effective HCP detection method. This kit uses the experimental principle of double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the residual amount of E. coli HCP for plasmids. Add the E. coli HCP standard for plasmids and the sample to be tested to the ELISA plate pre-coated with anti-E. coli HCP antibody for plasmids. Then add the diluted biotin-labeled E. coli HCP detection antibody for plasmids. Finally, horseradish peroxidase-labeled streptavidin (Streptavidin-HRP, SA-HRP) was added to form an antibody + antigen + antibody-Biotin + SA-HRP complex. After washing the plate, TMB colorimetric solution was added for color development. TMB was converted from colorless to blue under the catalysis of HRP enzyme and finally converted to yellow under the action of stop solution. The depth of yellow is positively correlated with the amount of E. coli HCP detected in the sample.

Please contact us at for specific academic pricing.