ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive)

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Background

ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive) is an enhanced chemiluminescence (ECL) substrate with ultra-high sensitivity and the most sensitive chemiluminescence substrate among our ECL luminescent solutions, achieving low femtogram levels (based on HRP concentration) through HRP. Western blot detection. Its sensitivity, luminous time, and background are noticeably excellent. It has the following characteristics:

(1) Sensitive: Femtogram-grade protein bands can be detected on nitrocellulose or PVDF membranes when appropriate primary and secondary antibodies are used.
(2) Quantification: The quantifiable range of the resulting signal spans two orders of magnitude.
(3) Bright signal: Exposure through film or imaging system for easy image capture.
(4) Long signal duration: Detectable optical signal output up to 8 hours under optimized conditions.
(5) Stabilized reagents: Kit components can be stable at 4°C for one year and at room temperature for 6 months.
(6) Affordable: Formulated to be optimized for very low concentration antibody detection.

When this product substrate is used in conjunction with optimized antibody concentrations and blocking buffers, low-abundance target proteins that cannot be detected by conventional ECL substrates can be detected.