SuperSignal ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive)

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Background

SuperSignal ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive) is an enhanced chemiluminescence (ECL) substrate with ultra-high sensitivity and an ECL luminescent liquid with ultra-long luminescence time of up to 16 h. HRP can achieve low femtogram (based on HRP concentration) western blot detection, and its sensitivity, luminescence time and background are obviously excellent.
It has the following characteristics:

(1) Sensitive: Femtogram-grade protein bands can be detected on nitrocellulose or PVDF membranes when appropriate primary and secondary antibodies are used.
(2) Quantification: The quantifiable range of the resulting signal spans two orders of magnitude.
(3) Bright signal: Exposure through film or imaging system for easy image capture.
(4) Long signal duration: Detectable optical signal output up to 16 hours under optimized conditions.
(5) Stabilized reagents: Kit components can be stable at 4°C for one year and at room temperature for 6 months.
(6) Affordable: Formulated to be optimized for very low concentration antibody detection.

When this product substrate is used in conjunction with optimized antibody concentrations and blocking buffers, low-abundance target proteins that cannot be detected by conventional ECL substrates can be detected.