DNase Viability Assay Kit (Fluorescent Labeling)

DNase Viability Assay Kit (Fluorescent Labeling)

Catalog Number:
EAK1505818YEA
Mfr. No.:
41322ES48/68
Price:
$553
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      • Overview
        • The DNase Activity Detection Kit (Fluorescent Labeling Method) uses a new type of DNA substrate, which is labeled with a fluorescent reporter group molecule at one end and a quencher group at the other end. In the absence of DNase, the physical proximity of the quencher group will quench the fluorescence in the fluorescent reporter group to an extremely low level. When DNase is present, the DNA substrate is hydrolyzed, and the fluorescent reporter group and the quencher group are spatially separated in the solution, which causes the fluorescent reporter group to emit bright fluorescence when excited by light of the appropriate wavelength, which can be detected by a multifunctional microplate reader (including a fluorescence module) and a fluorescent quantitative PCR instrument.

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      • Properties
        • Details
          Detection range: 1.25×10-6U/μL~1×10-5U/μL
          Detection method: Fluorescence labeling
          Detection duration: 1h
          Sensitivity: 1.25×10-6U/μL
          Intra-board difference: <10%
          Inter-plate difference: <15%
          Storage
          Part Ⅰ, stored at -25~-15°C; Part Ⅱ, stored at room temperature. Valid for 1 year.
          Part Ⅰ, transported at -25~-15°C; Part Ⅱ, transported at room temperature. After receiving the goods, please check whether all components are complete and store them at the corresponding storage temperature immediately.
          The validity period of the unopened kit is 1 year, and the validity period after opening is 6 months. It is recommended to divide the A component solution according to the amount used each time to avoid repeated freezing and thawing to affect the quality.

          * For Research Use Only

      • Applications
        • Application Description
          The DNase Activity Detection Kit (Fluorescent Labeling Method) uses a substrate fluorescent labeling method to quantitatively or qualitatively detect DNase in a single sample, thereby determining whether the sample is contaminated with DNase.

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