DNase Set

Bovine pancreatic deoxyribonuclease (also known as DNase) is an endonuclease. It acts on the phospholipid bond, especially the bond adjacent to pyrimidine nucleoside, to produce polynucleotides with free hydroxyl at the 3 'end and phosphate at the 5' end. The optimum pH value of is 7.8. DNase can be activated by divalent metals and inhibited by chelates such as EDTA and sodium dodecyl sulfate. 5mM calcium ion can be used as a stabilizer to protect DNase from being decomposed by hydrolase. This product is extracted from bovine pancreas and prepared by chromatography to remove the pollution of other hydrolases.

RNase Free DNase I Set is specially designed for column/magnetic bead RNA extraction kit. Biological samples were lysed, ethanol was added to adjust the binding conditions, and transferred to column or magnetic beads to adsorb RNA. After washing, add DNase I and DNase Buffer to the membrane of the column or magnetic bead, digest at room temperature (25-37°C) for 15 minutes to completely remove the DNA adsorbed on the membrane/beads, after wash away DNase and degraded DNA, and finally remove RNA with DEPC water.

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