DNA/RNA/Protein Extraction Kit

The Presto™ DNA RNA Protein Extraction Kit provides an efficient method for purifying genomic DNA, total RNA and total protein simultaneously from cultured cells, animal tissue, whole blood and biological fluids such as serum and plasma. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the genomic DNA mini spin column. The flow-through containing RNA can then be transferred to the RNA mini spin column for RNA binding. The proteins in the flow-through can then be precipitated using acetone. Contaminants are removed using a wash buffer and the purified genomic DNA is eluted in a low salt Elution Buffer and the purified total RNA is eluted in RNase-free Water. The entire procedure can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-time PCR, northern blotting, primer extension, mRNA selection and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.

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