DMNPE-caged Luciferin -for Transfection Assay

The cell permeable DMNPE-caged Luciferin is as a bioluminescent luciferase substrate used to measure intracellular functions since it readily crosses cell membranes. It is a D-luciferin ester analogue of luciferin.Once the caged luciferin is inside the cells, active luciferin can be released by the action of endogenous intracellular esterases or by a flash of UV light.
This implies:
A more efficient delivery of luciferin into intact living cells& improved sensitivity analysis of in vivo luciferase assays
A long-term measurement of luciferase activity since hydrolysis by esterases allows a continuous supply of active Luciferin
DMNPE-caged luciferin appears a good alternative to D-Luciferin classically used to measure luciferase activity in live cells. Indeed, the difficulty to deliver D-Luciferin into living cells renders in vivo quantification limited to a certain extent - for example, permeability issues at neutral pH.
High purity
Cell permeable: efficient luciferin delivery into intact cells even at neutral pH
Ideal for in vivo applications
Induces prolonged release of luciferin by the action of cells’ esterases
Allows to follow changes in gene expression in live cells
Suitable for in vitro experiments

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