G418 Sulfate -for Transfection Assay

G418 Sulfate -for Transfection Assay

Catalog Number:
TR02108564OZB
Mfr. No.:
GS21000
Price:
$279
  • Size:
    1 g
    Quantity:
    Add to Cart:
      • Overview
        • OZ Biosciences G418 Sulfate is an aminoglycoside antibiotic identical to gentamicin B1 produced by Micromonospora rhodorangea. It blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. It is used to select and maintain eukaryotic cells expressing the neo gene (neomycin). The quality and purity of the G-418 is essential to achieve good and consistent selection.
          Chemical Name: (2R,3S,4R,5R,6S)-5-amino-6-[(1R,2S,3S,4R,6S)-4,6-diamino-3-[(2R,3R,4R,5R)-3,5-dihydroxy-5-methyl4 methylaminooxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-2-(1-hydroxyethyl)oxane-3,4-diol
          Molecular Formula: C20H40N4O10. 2 H2SO4
          Molecular weight: 692.71 g/mol
          CAS Number: 108321-42-2
          Molecular biology grade and premium pure
          Size: 1g

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      • Properties
        • Categories
          Transfection Tools
          Storage
          -20°C
          Shipping
          Room temperature
      • Applications
        • Application Description
          RECOMMENDED FOR: selection and maintenance of cells expressing the neo gene. Productiong and maintaining stably-transfected cell lines.
          The G-418 sulfate blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. It is used to select and maintain eukaryotic cells expressing the neo gene (neomycin). G-418 resistance conferred by the neo gene encoding an aminoglycoside 3’-phosphotransferase, APH 3’ II is due to the inactivation of G-418 by covalent modification of its amino or hydroxyl functions. In contrast to gentamicin, G-418 blocks protein synthesis in eukaryotic cells by irreversibly binding to ribosomes and disrupting their proofreading capacity.

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