Direct cGMP ELISA

This is a colorimetric competitive immunoassay kit for the quantitation of intracellular cGMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution.

Sensitivity increased by acetylation (reagents included)
Ready-to-use liquid color-coded reagents reduce error
Absorbance read at 405 nm

Cited sample types include cerebral spinal fluid1 and microdialysate2.1.Increased cerebrospinal fluid cAMP levels in Alzheimer's disease: M. Martinez, et al. ; Brain Res. 846, 265 (1999)2. Mechanism underlying diuretic effect of L-NAME at a subpressor dose: M. Liang, et al. ; Ren. Physiol. 281, F414 (2001).

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