Direct cAMP ELISA

This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution.

Sensitivity increased 10-fold by acetylation (reagents included)
Absorbance is read at 405 nm
Large signal-to-background ratio offers superior sensitivity

This kit lets you easily assess adenylyl cyclase activation by GPCRs. Cited sample types include cell culture supernatant1, and tissue culture2.
1. Effect of Tumor Necrosis Factor-{{alpha}}, Interferon-{{gamma}}, and Transforming Growth Factor-{beta} on Adipogenesis and Expression of Thyrotropin Receptor in Human Orbital Preadipocyte Fibroblasts: R. W. Valyasevi, et al.; J. Clin. Endocrinol. Metab. 86, 903 (2001)2. Cyclic Adenosine Monophosphate Regulation of Ion Transport in PorcineVocal Fold Mucosae: M. Sivasankar, et al.; Laryngoscope 118, 1511 (2008)

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