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Overview
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DHE (Dihydroethidium) can freely penetrate the living cell membrane into the cell and be oxidized by ROS in the cell to form ethidium oxide; ethidium oxide can be incorporated into chromosomal DNA to produce red fluorescence. The amount and changes of cellular ROS content can be determined based on the generation of red fluorescence in living cells. DHE is mainly oxidized by superoxide anion ROS in cells and can be directly observed using a flow cytometer or fluorescence microscope. It is a quick and easy classic method for detecting ROS in tissues or cultured living cells.
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