Name: Fluo-4 AM, Calcium Ion Fluorescent Probe
Price: 244.00 USD

- Overview
  - Fluo-4 is a calcium fluorescent probe that replaces the Cl ions in the Fluo-3 structure with F ions. Since the Cl ions are replaced with F ions with stronger electron attraction, its maximum excitation wavelength will deviate by about 10 nm toward a shorter wavelength. This wavelength is closer to the wavelength of an argon laser, so when excited by an argon laser, the fluorescence intensity of Fluo-4 is stronger than that of Fluo-3.
    
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- Properties
  - Categories
    
    Subcellular Structure Fluorescent Dyes
    
    Other Properties
    
    Validity: 12 months
    Fluo-4 AM excitation /emission wavelength: 494 / 516nm
    
    Storage
    
    -20°C, keep away from light
    
    Shipping
    
    Shipped with ice pack
    
    Note
    
    · If a culture medium containing serum is used, the esterase in the serum will decompose the AM ester body, thereby reducing the effect of Fluo-4, AM ester entering the cells. In addition, a culture medium containing phenol red will slightly increase the background value. Before adding the working solution, the residual culture medium should be removed as much as possible.
    · All fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching.
    · Fluo-4, AM ester is easy to absorb moisture. After taking it out of the refrigerator, please make sure it is in a dry environment and put it at room temperature before opening. Since the reagent is very small, please centrifuge it briefly before opening to ensure that the powder falls to the bottom of the tube.
    · Fluo-4, AM ester is easily decomposed when in contact with water. If it cannot be used up all at once, it is recommended to store the stock solution in small portions.
    
    * For research use only
- Applications
  - Application Description
    
    Subcellular structure fluorescent dyes are fluorescent markers used to label and observe various organelles and structures within cells. They are of significant importance to cell biology research. These dyes provide powerful tools for cell biologists, enabling the visualization of different structures and functions within cells through appropriate selection and application.

Documents

Datasheet

Fluo-4 AM, Calcium Ion Fluorescent Probe

Fluo-4 AM, Calcium Ion Fluorescent Probe

Documents

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