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Overview
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Digitonin is produced from the seeds of the Digitalis pupurea and other Digitalis species. It is a steroid glycoside consisting of the aglycon digitogenin linked to five sugar residues. Digitonin reacts hemolytic and is toxic to humans. The reaction of naturally occurring steroids carrying a 3b-hydroxyl group with digitonin results in an insoluble precipitate. Determination of cholesterol and ergosterol. Digitonin permeabilizes the plasma membranes of eukaryotic cells by complexing with membrane cholesterol and other unconjugated and beta;-hydroxysterols. Used for solubilization of membrane proteins and permeabilization of cellular and nuclear membranes.
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