Diamond Butyl - Butyl HIC Resins

Diamond Butyl - Butyl HIC Resins

Catalog Number:
CM01502233BES
Mfr. No.:
AH0111-AH303316
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      • Overview
        • Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
          Diamond Butyl is formed by coupling weakly hydrophobic aliphatic butyl groups on high-rigidity agarose matrix. It has low back pressure and fast flow velocity, which is suitable for large-scale purification of highly hydrophobic biomolecules.

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      • Properties
        • Categories
          Butyl HIC Resins
          Other Properties
          Appearance: White slurry
          Matrix: Highly cross-linked rigid agarose
          Functional group: Butyl
          Ligand concentration: ~53μmol Ligand/mL resin
          Dynamic binding capacity: ~27mg BSA/mL packed resin
          Particle size +: 40~120μm
          Max. pressure: 0.5MPa
          Pressure flow velocity: ≥1200cm/h (0.5MPa BXK 100/500, H=20cm, 20°C)
          Chemical stability: Stable in common aqueous buffers: 1M NaOH++,1M HAc++,6M GuHCl,30% isopropyl alcohol,70% ethanol
          pH stability: 2~14 (CIP),3~13 (working)
          Operating temperature: Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C autoclaving (20min)
          Storage+++: 2~30°C,20% ethanol or 2% benzyl alcohol
          Recommended flow velocity: 150~350cm/h

          +Particle size is normally distributed, and particles within this range account for more than 75% of the total.
          ++1M NaOH and 1M HAc only be used for cleaning.
          +++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

          * For Research Use Only

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