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Overview
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Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
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Overview
Desthiobiotin-PEG3-NHS ester
Desthiobiotin-PEG3-NHS ester
Catalog Number:
PG01492653ALA
Mfr. No.:
D597253-100mg; D597253-250mg; D597253-500mg
Price:
$1,262
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