Deoxynivalenol ELISA Kit 96 Well Plate

The Deoxynivalenol ELISA allows the quantitative determination of Deoxynivalenol in food and feed crop (cereals). For further applications the user is encouraged to verify the validity of the tests themselves or contact the manufacturer.
The assay is carried out in a microplate, of which the cavities have already been pre-coated with a special anti- Deoxynivalenol antibody.
To carry out the assay, Deoxynivalenol (DON) standards and sample extracts respectively are pipetted into the cavities. After 15 min incubation the Deoxynivalenol peroxidase conjugate (DON-HRP-conjugate) is added without a preceding washing step.
Deoxynivalenol from the standard or sample competes with the HRP labelled Deoxynivalenol for the antibody binding sites. The unbound components are removed by rinsing them off and then peroxidase substrate solution (3,3’,5,5’-Tetramethylbenzidine, TMB) is pipetted into all cavities. The DON-HRP conjugate that is bound to the antibody reacts with the substrate solution by forming a blue colour. After 15 minutes, this reaction is terminated by adding the stop solution. The colour then changes from blue to yellow, which is detected photometrically. With the help of a microplate reader, the yellow colouring is measured as an optical density (OD) at a wavelength of 450nm (reference value 620nm).
The Deoxynivalenol concentration is inversely proportional to the colour intensity. The higher the OD measurement, the lower is the concentration of Deoxynivalenol in the standard or sample.

Assay time 45 min (15+15+15 min)
Lower detection limit: Wheat flour 12 ppb; oats 15 ppb
Cross reactivity: 15-Acetyldeoxynivalenol >100 %; 3-Acetyldeoxynivalenol <1 %; T2-Toxin <1 %; Nivalenol <1 %;
Storage: 2-8 °C

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