Human PCSK9 ELISA, 96 Well

The Human PCSK9 ELISA provides a sensitive and specific quantitative determination of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) in human serum samples. One kit contains reagents for 96 determinations, thus allowing the measurement of one standard curve and 40 samples in duplicate. The calibration curve covers the range from 5.27 ng/ml to 60 ng/ml. The sensitivity of the assay is 1 ng/ml. The recommended dilution of the serum samples is 1:20. The complete incubation time is 2 hours 20 minutes.
The wells of the microplate are pre-coated with a specific capture antibody against human PCSK9 that binds free PCSK9 from the patient sample. The analyte is detected in two steps using a biotin-labelled monoclonal antibody and a highly polymerised streptavidin-peroxidase conjugate. The incubation steps take place for 45 min/20 min at 20-25 °C. After a washing step, the colour substrate TMB (Tetramethylbenzidine) is added to the wells. The reaction is stopped by adding the stop solution and the yellow colour is read in a microtiter plate reader at 450 nm. The concentration of PCSK9 in a sample is determined by interpolation from the standard curve.

Sensitivity: The minimum detectable dose of human PCSK9 is 1 ng /ml.
The assay should be conducted with a serum dilution of 1:20. For dilutions of 1:20 to 1:40, the linearity of the assay results to their dilution is assured.

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