Human RBM3 ELISA, 96 Well

The Human RBM3 ELISA provides a fast, highly sensitive and specific quantitative determination of RNA-Binding Protein 3 (RBM3) in human serum or plasma samples. Do not use for any other biological sample. One kit contains reagents for 96 determinations, thus allowing the measurement of one standard curve and 40 samples in duplicate. The calibration curve covers the range from 31.25 pg/ml to 2000 pg/ml. The sensitivity of the assay is 10 pg/ml. The recommended dilution of the serum or plasma samples is 1:10. The complete incubation time is 2 hours 30 minutes.
The wells of the microplate are pre-coated with a specific capture antibody against human RBM3 that binds free RBM3 from the patient sample. In one step simultaneous incubation of the RBM3 standards or samples and of the detection conjugate (peroxidase conjugate of an anti-RBM3 antibody) is carried out. Incubation takes place for 2 hours at 20-25 °C. After a washing step, the colour substrate TMB (tetramethylbenzidine) is added to the wells. The reaction is stopped by adding the stop solution and the yellow colour is read in a microtiter plate reader at 450 nm. The concentration of RBM3 in a sample is determined by interpolation from the standard curve.
The minimum detectable dose of human RBM3 is less than 10 pg /ml.
The assay should be conducted with a serum dilution of 1:10. For dilutions of 1:20 to 1:30, the linearity of the assay results to their dilution is assured.

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